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Foundations of Preclinical Optical and Acoustic Imaging

Introduction to Preclinical and In Vivo Imaging

Comparison of Optical and Photoacoustic Imaging Modalities

Light Transport in Biological Tissue

Preclinical Study Design, Animal Preparation, and Safety

Bioluminescence Imaging for Whole-Animal Studies

Core Principles of Bioluminescence Imaging

BLI Instrumentation and Image Acquisition

BLI Quantification, Interpretation, and Limitations

Near-Infrared Fluorescence Imaging: NIR-I and NIR-II

Principles of Near-Infrared Fluorescence Imaging

NIR-I Whole-Animal Imaging

NIR-II Whole-Animal Imaging

NIR Imaging Workflow and Quantitative Analysis

Photoacoustic Microscopy and Tomography

Photoacoustic Signal Generation and Hybrid Contrast

Photoacoustic Microscopy

Photoacoustic Tomography and Photoacoustic Computed Tomography

Functional and Multispectral Photoacoustic Imaging

Photoacoustic Image Reconstruction, Quality Control, and Interpretation

Modality Selection and Integrated Preclinical Applications

Selecting an Imaging Modality for a Research Question

Case Studies in Longitudinal Whole-Animal Imaging

Quiz

Quiz Question 1: Optical and Photoacoustic Modalities

Quiz Question 2: Bioluminescence Imaging

Quiz Question 3: NIR-I and NIR-II Imaging

Quiz Question 4: Photoacoustic Microscopy and Tomography

Quiz Question 5: Modality Selection

Survey and Feedback

Course Feedback Survey

Welcome to "Principles of Preclinical Optical and Photoacoustic Imaging," a comprehensive online course designed to equip researchers, scientists, and students with the foundational knowledge and practical skills required for cutting-edge in vivo imaging. Preclinical imaging plays a pivotal role in modern biomedical research, enabling longitudinal studies that reduce animal use while providing deep insights into tumor progression, cell tracking, and therapeutic responses.

Welcome to "Principles of Preclinical Optical and Photoacoustic Imaging," a comprehensive online course designed to equip researchers, scientists, and students with the foundational knowledge and practical skills required for cutting-edge in vivo imaging. Preclinical imaging plays a pivotal role in modern biomedical research, enabling longitudinal studies that reduce animal use while providing deep insights into tumor progression, cell tracking, and therapeutic responses. This course dives deep into the core modalities of optical and photoacoustic imaging, offering a thorough comparison of their strengths, limitations, and applications.

Throughout this program, you will explore the mechanics of light transport in biological tissues and master the principles of Bioluminescence Imaging (BLI) and Near-Infrared (NIR-I and NIR-II) Fluorescence Imaging. Furthermore, you will uncover the innovative world of Photoacoustic Microscopy (PAM) and Photoacoustic Computed Tomography (PACT), learning how hybrid contrast mechanisms combine optical sensitivity with acoustic depth resolution.

By the end of the course, you will be adept at designing robust preclinical studies, selecting the optimal imaging modality for your specific research questions, and performing quantitative image analysis. Whether you are investigating microvascular structures, monitoring targeted probe biodistribution, or evaluating tumor hypoxia, this course provides the theoretical framework and practical case studies needed to elevate your preclinical research. Join us to master the techniques that are driving the future of noninvasive whole-animal imaging.

Principles of Preclinical Optical and Photoacoustic Imaging

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            <p>Preclinical in vivo imaging serves as a critical methodology in contemporary biomedical research, facilitating the noninvasive and longitudinal assessment of biological processes within small-animal models. The course, Principles of Preclinical Optical and Photoacoustic Imaging, offers a rigorous exploration of the theoretical foundations, technical instrumentation, and practical applications of these sophisticated imaging modalities. By examining the distinct mechanisms of signal generation and light-tissue interactions, participants will gain a profound understanding of how optical and acoustic techniques can be leveraged to extract molecular, functional, and anatomical information while optimizing experimental design and animal welfare.</p>

<p>The curriculum is systematically structured to guide learners from foundational concepts to advanced multimodal applications. Initial modules establish the principles of light transport in biological tissue, alongside essential protocols for study design, animal preparation, and safety. Subsequent sections provide in-depth analyses of specific modalities, including Bioluminescence Imaging (BLI), Near-Infrared (NIR-I and NIR-II) fluorescence imaging, and Photoacoustic Microscopy and Tomography (PAM and PACT). Through detailed examinations of image acquisition workflows, quantitative analysis, and multispectral reconstruction, the course equips researchers with the analytical skills necessary to mitigate artifacts and interpret complex data. Ultimately, integrated case studies and modality selection frameworks will empower investigators to design robust, longitudinal experiments tailored to their specific scientific inquiries.</p>
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